The Effect of Two Different Extraction Methods for Antioxidant Activity on Petroleum Ether Extract of Leaves of Plant Casearia Tomentosa

3 Department of Chemistry, Gurukula Kangri Vishwavidyalaya, Haridwar, Uttarakhand, (India) ABSTRACT In the present study the effect of maceration and soxhlet extraction from plant leaves of Casearia tomentosa were compared for phytochemical screening and antioxidant activity using petroleum ether as solvent. The phytochemical screening was evaluated using standard protocols and the antioxidant activity was performed by 1,1-diphenyl-2picrylhydrazyl (DPPH) free radical scavenging method. The results of preliminary phytochemical screening in both extracts revealed the presence of terpenoids, steroids, phytosterol, fat and oil etc. But the extract obtained from soxhlet method showed higher antioxidant activity (IC50 value 280 μg/ml) than maceration (IC50 value 480 μg/ml). All these experimental analysis shows that the Casearia tomentosa leaves could be a potential source of natural antioxidant and may be used to prevent oxidative stress. KeywordsAntioxidant activity, Casearia tomentosa, Free radical scavenging, Phytochemical, Terpenoids etc.